Application
Fig1: Western blot analysis of E Cadherin on MCF-7(1) and A431 (2) cells lysates using anti-E-Cadherin antibody at 1/1,000 dilution.Application
Fig2: ICC staining E Cadherin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.Application
Fig3: ICC staining E Cadherin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.Application
Fig4: ICC staining E Cadherin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-E Cadherin antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of A431 cells with E Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | E Cadherin [SY0287] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arc 1 antibody CADH1_HUMAN antibody Cadherin 1 antibody cadherin 1 type 1 E-cadherin antibody Cadherin1 antibody CAM 120/80 antibody CD 324 antibody CD324 antibody CD324 antigen antibody cdh1 antibody CDHE antibody E-Cad/CTF3 antibody E-cadherin antibody ECAD antibody Epithelial cadherin antibody epithelial calcium dependant adhesion protein antibody LCAM antibody Liver cell adhesion molecule antibody UVO antibody Uvomorulin antibody |
| Product description | Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Arc 1 antibody CADH1_HUMAN antibody Cadherin 1 antibody cadherin 1 type 1 E-cadherin antibody Cadherin1 antibody CAM 120/80 antibody CD 324 antibody CD324 antibody CD324 antigen antibody cdh1 antibody CDHE antibody E-Cad/CTF3 antibody E-cadherin antibody ECAD antibody Epithelial cadherin antibody epithelial calcium dependant adhesion protein antibody LCAM antibody Liver cell adhesion molecule antibody UVO antibody Uvomorulin antibody |
|---|---|
| Molecular Weight(MW) | 97/91 kDa |
| Cellular Localization | Cell junction, Cell membrane, Endosome, Golgi apparatus. |
| SwissProt ID | P12830 |
|---|
Application
Fig1: Western blot analysis of E Cadherin on MCF-7(1) and A431 (2) cells lysates using anti-E-Cadherin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining E Cadherin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.
Application
Fig3: ICC staining E Cadherin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.
Application
Fig4: ICC staining E Cadherin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-E Cadherin antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A431 cells with E Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, MCF-7, A549, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
