Application
Fig1: Western blot analysis of Bak on different lysates using anti-Bak antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Human skeletal muscle Lane 3: AgsApplication
Fig2: ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Bak [SU32-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis regulator BAK antibody BAK antibody BAK like antibody Bak NT antibody BAK_HUMAN antibody Bak1 antibody Bcl 2 homologous antagonist/killer antibody Bcl 2 like 7 protein antibody Bcl-2 homologous antagonist/killer antibody Bcl-2-like protein 7 antibody BCL2 antagonist/killer 1 antibody Bcl2 like 7 Protein antibody Bcl2-L-7 antibody BCL2L7 antibody CDN1 antibody Cell death inhibitor 1 antibody MGC117255 antibody MGC3887 antibody NBak antibody Pro apoptotic protein BAK antibody |
| Product description | The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis, while other members of the Bcl-2 family, such as Bax, accelerate death under various conditions. One member of the Bcl-2 family, designated Bak, functions primarily to enhance apoptotic cell death following appropriate activating signals and counteracts the protection from apoptosis provided by Bcl-2. Expression of Bak is widespread in a broad range of cells, including various long-lived, terminally differentiated cell types, suggesting that its cell-death-inducing activity is broadly distributed and that the regulation of inhibitors of apoptosis may represent an important determinant of tissue-specific modulation of apoptosis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis regulator BAK antibody BAK antibody BAK like antibody Bak NT antibody BAK_HUMAN antibody Bak1 antibody Bcl 2 homologous antagonist/killer antibody Bcl 2 like 7 protein antibody Bcl-2 homologous antagonist/killer antibody Bcl-2-like protein 7 antibody BCL2 antagonist/killer 1 antibody Bcl2 like 7 Protein antibody Bcl2-L-7 antibody BCL2L7 antibody CDN1 antibody Cell death inhibitor 1 antibody MGC117255 antibody MGC3887 antibody NBak antibody Pro apoptotic protein BAK antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Mitochondrion membrane. |
| SwissProt ID | Q16611 |
|---|
Application
Fig1: Western blot analysis of Bak on different lysates using anti-Bak antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Human skeletal muscle Lane 3: Ags
Application
Fig2: ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Ags, CRC, Hela, human lung tissue, mouse lung tissue, human skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
