ICC/IF
ICC staining Phospho-IRF3(S386) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.WB
Western blot analysis of Phospho-IRF3 (S386) on different lysates with Rabbit anti-Phospho-IRF3 (S386) antibody at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate, Lane 2: MCF7 treated with 100nM Calyculin A for 30 minutes whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-IRF3 (S386) on different lysates with Rabbit anti-Phospho-IRF3 (S386) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-IRF3 (S386) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP). |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser386 of human IRF3. |
| Modification | p-S386 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | IIAE7 antibody Interferon regulatory factor 3 antibody IRF 3 antibody IRF-3 antibody IRF3 antibody IRF3_HUMAN antibody MGC94729 antibody |
|---|---|
| Molecular Weight(MW) | 47kDa(Observed band size: 55kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q14653 |
|---|
ICC/IF
ICC staining Phospho-IRF3(S386) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
WB
Western blot analysis of Phospho-IRF3 (S386) on different lysates with Rabbit anti-Phospho-IRF3 (S386) antibody at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate, Lane 2: MCF7 treated with 100nM Calyculin A for 30 minutes whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-IRF3 (S386) on different lysates with Rabbit anti-Phospho-IRF3 (S386) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Positive Control | MCF-7. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
