Application
Fig1: Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431Application
Fig2: ICC staining Cytokeratin 8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cytokeratin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with Cytokeratin 8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Cytokeratin 8 [SU0338] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CARD2 antibody Cell proliferation inducing gene 46 protein antibody Cell proliferation inducing protein 46 antibody CK 8 antibody CK-8 antibody CK8 antibody CYK8 antibody Cytokeratin 8 antibody Cytokeratin-8 antibody K2C8 antibody K2C8_HUMAN antibody K8 antibody Keratin 8 antibody keratin type II cytoskeletal 8 antibody Keratin-8 antibody KRT8 antibody type II cytoskeletal 8 antibody Type-II keratin Kb8 antibody |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CARD2 antibody Cell proliferation inducing gene 46 protein antibody Cell proliferation inducing protein 46 antibody CK 8 antibody CK-8 antibody CK8 antibody CYK8 antibody Cytokeratin 8 antibody Cytokeratin-8 antibody K2C8 antibody K2C8_HUMAN antibody K8 antibody Keratin 8 antibody keratin type II cytoskeletal 8 antibody Keratin-8 antibody KRT8 antibody type II cytoskeletal 8 antibody Type-II keratin Kb8 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Nucleus matrix. |
Application
Fig1: Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431
Application
Fig2: ICC staining Cytokeratin 8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cytokeratin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with Cytokeratin 8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, MCF-7, Hela, HepG2, human liver tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
