Application
Fig1: Western blot analysis of CD31 on THP-1 cell lysates using anti-CD31 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of THP-1 cells with CD31 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CD31 [SU03-59] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adhesion molecule antibody CD31 antibody CD31 antigen antibody CD31 EndoCAM antibody EndoCAM antibody FLJ34100 antibody FLJ58394 antibody GPIIA antibody GPIIA' antibody PECA1 antibody PECA1_HUMAN antibody Pecam 1 antibody PECAM 1 CD31 EndoCAM antibody PECAM antibody PECAM-1 antibody Pecam1 antibody Platelet and endothelial cell adhesion molecule 1 antibody Platelet endothelial cell adhesion molecule antibody Platelet/endothelial cell adhesion molecule 1 antibody |
| Product description | Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms’ tumors, Ewing’s sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the β2/α1 and β2/αM integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM)-like protein and is deleted in 66% of patients with Kallmann’s syndrome, anosmia with secondary hypogonadism. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200: FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Adhesion molecule antibody CD31 antibody CD31 antigen antibody CD31 EndoCAM antibody EndoCAM antibody FLJ34100 antibody FLJ58394 antibody GPIIA antibody GPIIA' antibody PECA1 antibody PECA1_HUMAN antibody Pecam 1 antibody PECAM 1 CD31 EndoCAM antibody PECAM antibody PECAM-1 antibody Pecam1 antibody Platelet and endothelial cell adhesion molecule 1 antibody Platelet endothelial cell adhesion molecule antibody Platelet/endothelial cell adhesion molecule 1 antibody |
|---|---|
| Molecular Weight(MW) | 82kDa(Observed: 130 kDa) |
| Cellular Localization | Cell junction. Cell membrane. Membrane. |
| SwissProt ID | P16284 |
|---|
Application
Fig1: Western blot analysis of CD31 on THP-1 cell lysates using anti-CD31 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of THP-1 cells with CD31 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | THP-1, human tonsil tissue, human kidney tissue, human uterus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200: FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
