Application
Fig1: Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: HEK-293 cell lysate (15 µg/Lane), Lane 3: A549 cell lysate (15 µg/Lane) Exposure time: 1 minute 36 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig10:Flow cytometric analysis of Hela cells with Lactate Dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Application
Fig11:Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.| Product Name | Lactate Dehydrogenase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The lactate dehydrogenase family (LDH) catalyzes the final step of anaerobic glycolysis, the conversion of L-lactate and NAD to pyruvate and NADH. The LDH family consists of three members, LDH-A, LDH-B and LDH-C, all of which form tetramers consisting four subunits. However, each family member displays a specific tissue distribution pattern with LDH-A and LDH-B predominant in several tissues, specifically LDH-A in muscle and LDH-B in heart, while LDH-C expression is confined to the testis and sperm. LDHs function as powerful markers for germ cell tumors. The genes encoding human LDH-A and LDH-C map to chromosome 11, while the human LDH-B gene maps to chromosome 12. Deficiency in the LDH-A gene is linked to exertional myoglobinuria. |
| Immunogen | Synthetic peptide within human LDHB aa 270-310. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:5000 ICC/IF:1:50-1:200 IHC:1:200-1:2000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Cell proliferation-inducing gene 19 protein antibody GSD11 antibody L lactate dehydrogenase B chain antibody L-lactate dehydrogenase A chain antibody Lactate dehydrogenase A antibody Lactate dehydrogenase B antibody Lactate dehydrogenase H chain antibody Lactate dehydrogenase M antibody LDH A antibody LDH B antibody LDH H antibody LDH heart subunit antibody LDH M antibody LDH muscle subunit antibody LDH-A antibody LDH-M antibody LDH1 antibody ldha antibody LDHA_HUMAN antibody LDHBD antibody LDHM antibody MS1111 antibody PIG19 antibody Proliferation inducing gene 19 antibody Renal carcinoma antigen NY REN 46 antibody Renal carcinoma antigen NY-REN-59 antibody TRG 5 antibody TRG5 antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: HEK-293 cell lysate (15 µg/Lane), Lane 3: A549 cell lysate (15 µg/Lane) Exposure time: 1 minute 36 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig10:Flow cytometric analysis of Hela cells with Lactate Dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Fig11:Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.| Application Notes | WB:1:1000-1:5000 ICC/IF:1:50-1:200 IHC:1:200-1:2000 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
