Application
Fig1: Western blot analysis of Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) on different lysates using anti-Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: SH-SY-5YApplication
Fig2: ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of NIH/3T3 cells with Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cytoplasm, Cell membrane. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser144 of Human PAK1 aa 121-150 / 545. |
| Modification | p-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Molecular Weight(MW) | 61kDa |
|---|---|
| Cellular Localization | Cytoplasm, Cell membrane. |
Application
Fig1: Western blot analysis of Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) on different lysates using anti-Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: SH-SY-5Y
Application
Fig2: ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of NIH/3T3 cells with Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NIH/3T3, SH-SY-5Y, Hela, human liver cancer tissue, mouse brain tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
