WB
Western blot analysis of Cleaved+pro Caspase-3 on different lysates with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate, Lane 3: HeLa treated with 3μM staurosporine for 4 hours cell lysate, Lane 4: C6 cell lysate, Lane 5: C6 treated with 1μM staurosporine for 4 hours cell lysate, Lane 6: C6 treated with 3μM staurosporine for 4 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Cleaved+pro Caspase-3 on different lysates with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/5,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: MDA-MB-231 cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: Jurkat cell lysate, Lane 5: Jurkat treated with 1μM staurosporine for 3 hours cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Cleaved+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system. |
| Immunogen | Recombinant protein within Human Caspase-3 aa 9-175 / 277. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:2000 IHC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Apopain antibody CASP 3 antibody CASP-3 antibody CASP3 antibody CASP3_HUMAN antibody Caspase 3 antibody Caspase-3 subunit p12 antibody CPP 32 antibody CPP-32 antibody CPP32B antibody Cysteine protease CPP32 antibody PARP cleavage protease antibody Protein Yama antibody SCA-1 antibody SCA1 antibody SREBP cleavage activity 1 antibody Yama antibody |
|---|---|
| Molecular Weight(MW) | 32/17 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P42574 |
|---|
WB
Western blot analysis of Cleaved+pro Caspase-3 on different lysates with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate, Lane 3: HeLa treated with 3μM staurosporine for 4 hours cell lysate, Lane 4: C6 cell lysate, Lane 5: C6 treated with 1μM staurosporine for 4 hours cell lysate, Lane 6: C6 treated with 3μM staurosporine for 4 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Cleaved+pro Caspase-3 on different lysates with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/5,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: MDA-MB-231 cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: Jurkat cell lysate, Lane 5: Jurkat treated with 1μM staurosporine for 3 hours cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Cleaved+pro Caspase-3 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:2000 IHC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
