WB
Western blot analysis of Myosin light chain kinase / MLCK on rat spleen tissue lysates with Rabbit anti-Myosin light chain kinase / MLCK antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue with Rabbit anti-Myosin light chain kinase / MLCK antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MYLK antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.| Product Name | Myosin light chain kinase / MLCK Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains. |
| Immunogen | Synthetic peptide within Human MLCK aa 1,865-1,914 / 1,914. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1000-1:10000 IHC:1:100-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | deglutamylated form antibody DKFZp686I10125 antibody EC 2.7.11.18 antibody FLJ12216 antibody Kinase related protein antibody Kinase-related protein antibody KRP antibody MLCK antibody MLCK1 antibody MLCK108 antibody MLCK210 antibody MSTP083 antibody MYLK antibody MYLK_HUMAN antibody MYLK1 antibody Myosin light chain kinase antibody Myosin light polypeptide kinase antibody OTTHUMP00000180642 antibody OTTHUMP00000180643 antibody smMLCK antibody smooth muscle antibody Smooth muscle myosin light chain kinase antibody Telokin antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Cytoplasm, Cell projection, Cleavage furrow. |
WB
Western blot analysis of Myosin light chain kinase / MLCK on rat spleen tissue lysates with Rabbit anti-Myosin light chain kinase / MLCK antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue with Rabbit anti-Myosin light chain kinase / MLCK antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MYLK antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.| Application Notes | WB:1:1000-1:10000 IHC:1:100-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
