WB
Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane), Lane 2: THP-1 cell lysate (20 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining MCSF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining MCSF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCSF antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-MCSF antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MCSF antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-M-CSF antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | M-CSF Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The macrophage colony-stimulating factor (M-CSF), also designated CSF-1, was originally discovered in serum, urine and other biological fluids as a factor that can stimulate the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. M-CSF is a homodimeric cytokine that is produced by fibroblasts, epithelial cells, bone marrow stromal cells, osteoblasts, keratinocytes, macrophages, T cells and B cells. M-CSF is a glycoprotein required for the proliferation and differentiation of mononuclear phagocytes, including osteoclasts. M-CSF has also been identified as an important mediator of the inflammatory response and can regulate the release of proinflammatory cytokines from macrophages. M-CSF exerts its pleiotropic effects by binding to a single type of high affinity cell surface receptor that is encoded by the c-Fms proto-oncogene. |
| Immunogen | Synthetic peptide within Human M-CSF aa 56-105 / 554. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Colony stimulating factor 1 (macrophage) antibody Colony stimulating factor 1 antibody Colony stimulating factor macrophage specific antibody CSF 1 antibody CSF-1 antibody CSF1 antibody CSF1_HUMAN antibody Csfm antibody Lanimostim antibody M CSF antibody M-CSF antibody Macrophage Colony Stimulating Factor 1 antibody Macrophage colony stimulating factor antibody MCSF antibody MGC31930 antibody Processed macrophage colony-stimulating factor 1 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane, Secreted. |
| SwissProt ID | P09603 |
|---|
WB
Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane), Lane 2: THP-1 cell lysate (20 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining MCSF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining MCSF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCSF antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-MCSF antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MCSF antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-M-CSF antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
