WB
Western blot analysis of PI 3 Kinase p85 beta on different lysates with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. Lane 1: Rat heart tissue lysate, Lane 2: PC-12 cell lysate, Lane 3: HeLa cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 3 minutes; Lane 3: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of HeLa cells labeling PI 3 Kinase p85 beta. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
PI 3 Kinase p85 beta was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-PI 3 Kinase p85 beta antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PI 3 Kinase p85 beta antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-PI 3 Kinase p85 beta antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PI 3 Kinase p85 beta antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes.| Product Name | PI 3 Kinase p85 beta Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110α expression is restricted to white blood cells. It has been shown to bind p85α and p85β, but it apparently does not phosphorylate these subunits. p110α seems to have the capacity to autophosphorylate. p110α does not interact with the p85 subunits. It has been shown to be activated by α and β heterotrimeric G proteins. |
| Immunogen | Synthetic peptide within Human PI 3 Kinase p85 beta aa 136-185 / 728. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1000-1:2000 ICC/IF:1:50-1:200 IHC:1:200-1:1000 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | p85 antibody
p85 beta antibody
P85B antibody
P85B_HUMAN antibody
Phosphatidylinositol 3 kinase antibody
Phosphatidylinositol 3 kinase regulatory beta subunit antibody
Phosphatidylinositol 3 kinase regulatory subunit beta antibody
Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody Phosphatidylinositol 3-kinase regulatory subunit beta antibody Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody Phosphoinositide 3 kinase regulatory subunit 2 antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody Phosphoinositide 3 kinase regulatory subunit 2 (p85 beta) antibody PI3 kinase p85 beta subunit antibody PI3 kinase p85 subunit beta antibody PI3-kinase regulatory subunit beta antibody PI3-kinase subunit p85-beta antibody PI3K antibody PI3K regulatory subunit beta antibody PIK3R 2 antibody PIK3R2 antibody PtdIns 3 kinase p85 beta antibody PtdIns-3-kinase regulatory subunit beta antibody PtdIns-3-kinase regulatory subunit p85-beta antibody |
|---|---|
| Molecular Weight(MW) | 82kDa |
| Cellular Localization | Cytosol, nucleus. |
WB
Western blot analysis of PI 3 Kinase p85 beta on different lysates with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. Lane 1: Rat heart tissue lysate, Lane 2: PC-12 cell lysate, Lane 3: HeLa cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 3 minutes; Lane 3: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of HeLa cells labeling PI 3 Kinase p85 beta. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
PI 3 Kinase p85 beta was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-PI 3 Kinase p85 beta antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PI 3 Kinase p85 beta antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-PI 3 Kinase p85 beta antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PI 3 Kinase p85 beta antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:50-1:200 IHC:1:200-1:1000 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
