Application
Fig1: Western blot analysis of Ki67 on HepG2 cell lysates using anti-Ki67 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.Application
Fig4: Flow cytometric analysis of Hela cells with Ki67 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Ki67 [ST50-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen identified by monoclonal antibody Ki 67 antibody Antigen identified by monoclonal antibody Ki-67 antibody Antigen KI-67 antibody Antigen KI67 antibody Antigen Ki67 antibody KI67_HUMAN antibody KIA antibody Marker of proliferation Ki-67 antibody MIB 1 antibody MIB antibody MKI67 antibody PPP1R105 antibody Proliferation marker protein Ki-67 antibody Proliferation related Ki 67 antigen antibody Protein phosphatase 1 regulatory subunit 105 antibody RP11-380J17.2 antibody |
| Product description | Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation. Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:1,000-1:2,000 IHC |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Antigen identified by monoclonal antibody Ki 67 antibody Antigen identified by monoclonal antibody Ki-67 antibody Antigen KI-67 antibody Antigen KI67 antibody Antigen Ki67 antibody KI67_HUMAN antibody KIA antibody Marker of proliferation Ki-67 antibody MIB 1 antibody MIB antibody MKI67 antibody PPP1R105 antibody Proliferation marker protein Ki-67 antibody Proliferation related Ki 67 antigen antibody Protein phosphatase 1 regulatory subunit 105 antibody RP11-380J17.2 antibody |
|---|---|
| Molecular Weight(MW) | 359 kDa |
| Cellular Localization | Nucleus, Chromosome. |
| SwissProt ID | P46013 |
|---|
Application
Fig1: Western blot analysis of Ki67 on HepG2 cell lysates using anti-Ki67 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Application
Fig4: Flow cytometric analysis of Hela cells with Ki67 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HepG2, Hela, human tonsil tissue, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
