Application
Fig1: ICC staining GRM5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining GRM5 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GRM5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of SH-SY-5Y cells with GRM5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Metabotropic glutamate receptor 5 [ST51-00] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glutamate receptor metabotropic 5 antibody GPRC1E antibody Grm5 antibody GRM5_HUMAN antibody Metabotropic glutamate receptor 5 antibody Metabotropic glutamate receptor 5 variant F antibody Metabotropic glutamate receptor 5 variant G antibody Metabotropic glutamate receptor 5 variant H antibody mGlu5 antibody mGluR5 antibody PPP1R86 antibody Protein phosphatase 1 regulatory subunit 86 antibody |
| Product description | The mGluR proteins (metabotropic Glutamate Receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic Glutamate Receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR1 and mGluR5 are mediated by a G protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight sub-types of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-5, which can interact with SIAH-1, RyR-1, RyR-2, ITPR1, Shank 1, Shank 3 and GRASP, acts as a receptor for glutamate. The PPXXf motif of mGluR-5 binds to HOM1, HOM2 and HOM3. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Glutamate receptor metabotropic 5 antibody GPRC1E antibody Grm5 antibody GRM5_HUMAN antibody Metabotropic glutamate receptor 5 antibody Metabotropic glutamate receptor 5 variant F antibody Metabotropic glutamate receptor 5 variant G antibody Metabotropic glutamate receptor 5 variant H antibody mGlu5 antibody mGluR5 antibody PPP1R86 antibody Protein phosphatase 1 regulatory subunit 86 antibody |
|---|---|
| Molecular Weight(MW) | 132 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: ICC staining GRM5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining GRM5 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GRM5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of SH-SY-5Y cells with GRM5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | JAR, PC12, SHG-44, rat brain tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
