Application
Fig1: Western blot analysis of ATP citrate lyase on different lysates using anti-ATP citrate lyase antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: Mouse colon Lane 3: MCF-7Application
Fig2: ICC staining ATP citrate lyase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ATP citrate lyase in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ATP citrate lyase citrate lyase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with ATP citrate lyase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ATP citrate lyase [ST51-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACL antibody Acly antibody ACLY_HUMAN antibody ATP citrate (pro-S) lyase antibody ATP citrate lyase antibody ATP citrate synthase antibody ATP-citrate (pro-S-)-lyase antibody ATP-citrate synthase antibody ATPcitrate synthase antibody ATPCL antibody Citrate cleavage enzyme antibody CLATP antibody OTTHUMP00000164773 antibody |
| Product description | ATP-citrate synthase, also designated ATP-citrate lyase or citrate cleavage enzyme, is a cytoplasmic homotetramer belonging to the succinate/malate CoA ligase family. The gene coding for this protein maps against chromosome 17q12-q21. ATP-citrate synthase catalyses the formation of acetyl-CoA and oxaloacetate from citrate and CoA. This product, Acetyl-CoA, is necessary for both fatty acid and cholesterol biosynthesis. ATP citrate-lyase is important in the biosynthesis of acetylcholine in nervous tissue. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ACL antibody Acly antibody ACLY_HUMAN antibody ATP citrate (pro-S) lyase antibody ATP citrate lyase antibody ATP citrate synthase antibody ATP-citrate (pro-S-)-lyase antibody ATP-citrate synthase antibody ATPcitrate synthase antibody ATPCL antibody Citrate cleavage enzyme antibody CLATP antibody OTTHUMP00000164773 antibody |
|---|---|
| Molecular Weight(MW) | 122 kDa |
| Cellular Localization | Cytoplasm, nucleoplasm. |
Application
Fig1: Western blot analysis of ATP citrate lyase on different lysates using anti-ATP citrate lyase antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: Mouse colon Lane 3: MCF-7
Application
Fig2: ICC staining ATP citrate lyase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ATP citrate lyase in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ATP citrate lyase citrate lyase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with ATP citrate lyase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, MCF-7, Hela, CRC, mouse kidney tissue, human thyroid tissue, human breast carcinoma tissue, mouse thyroid tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
