Application
Fig1: Western blot analysis of Phospho-BTK(Y223) on different lysates using anti-Phospho-BTK(Y223) antibody at 1/1,000 dilution. Positive control: Lane 1: K562 cells treated with pervanadate Lane 2: Untreated K562 cell lysate| Product Name | Phospho-BTK(Y223) [ST51-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Agammaglobulinaemia tyrosine kinase antibody AGMX 1 antibody AGMX1 antibody AT antibody ATK antibody B cell progenitor kinase antibody B-cell progenitor kinase antibody BPK antibody Bruton agammaglobulinemia tyrosine kinase antibody Bruton tyrosine kinase antibody Bruton’s Tyrosine Kinase antibody Btk antibody BTK_HUMAN antibody dominant-negative kinase-deficient Brutons tyrosine kinase antibody IMD 1 antibody IMD1 antibody MGC126261 antibody MGC126262 antibody OTTHUMP00000063593 antibody PSCTK 1 antibody PSCTK1 antibody truncated Bruton agammaglobulinemia tyrosine kinase antibody Tyrosine protein kinase BTK antibody Tyrosine-protein kinase BTK antibody tyrosine-protein kinase BTK isoform (lacking exon 14 antibody XLA antibody |
| Product description | Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton’s disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human BTK. |
| Modification | p-Y223 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIP |
| WB:1:1,000-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Agammaglobulinaemia tyrosine kinase antibody AGMX 1 antibody AGMX1 antibody AT antibody ATK antibody B cell progenitor kinase antibody B-cell progenitor kinase antibody BPK antibody Bruton agammaglobulinemia tyrosine kinase antibody Bruton tyrosine kinase antibody Bruton’s Tyrosine Kinase antibody Btk antibody BTK_HUMAN antibody dominant-negative kinase-deficient Brutons tyrosine kinase antibody IMD 1 antibody IMD1 antibody MGC126261 antibody MGC126262 antibody OTTHUMP00000063593 antibody PSCTK 1 antibody PSCTK1 antibody truncated Bruton agammaglobulinemia tyrosine kinase antibody Tyrosine protein kinase BTK antibody Tyrosine-protein kinase BTK antibody tyrosine-protein kinase BTK isoform (lacking exon 14 antibody XLA antibody |
|---|---|
| Molecular Weight(MW) | 76 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane. |
| SwissProt ID | Q06187 |
|---|
Application
Fig1: Western blot analysis of Phospho-BTK(Y223) on different lysates using anti-Phospho-BTK(Y223) antibody at 1/1,000 dilution. Positive control: Lane 1: K562 cells treated with pervanadate Lane 2: Untreated K562 cell lysate| Positive Control | Treated K562. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
