WB
Western blot analysis of PGK1 on different lysates with Rabbit anti-PGK1 antibody at 1/2,000 dilution. Lane 1: HT-29 cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: C2C12 cell lysate, Lane 4: C6 cell lysate, Lane 5: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | PGK1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis. |
| Immunogen | Synthetic peptide within Human PGK1 aa 1-49 / 417. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Cell migration-inducing gene 10 protein antibody Epididymis secretory sperm binding protein Li 68p antibody HEL S 68p antibody MGC117307 antibody MGC8947 antibody MIG10 antibody pgk1 antibody PGK1_HUMAN antibody PGKA antibody Phosphoglycerate kinase 1 antibody Primer recognition protein 2 antibody PRP 2 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of PGK1 on different lysates with Rabbit anti-PGK1 antibody at 1/2,000 dilution. Lane 1: HT-29 cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: C2C12 cell lysate, Lane 4: C6 cell lysate, Lane 5: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
