Application
Fig1: Western blot analysis of alpha+beta Synuclein on different lysates with Rabbit anti-alpha+beta Synuclein antibody at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Rat brain tissue lysate, Lysates/proteins at 40 µg/Lane. Predicted band size: 14 kDa Observed band size: 15 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining alpha+beta Synuclein in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining alpha+beta Synuclein in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-alpha+beta Synuclein antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha+beta Synuclein antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with alpha+beta Synuclein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | alpha+beta Synuclein Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The synucleins, including α-synuclein (also designated NACP for nonamyloid component precursor), β-synuclein (also designated PNP 14 for phospho-neuroprotein 14) and γ-synuclein (also designated persyn or BCSG1 for breast cancer-specific gene 1) are presynaptic proteins abundant in neurons. Synucleins are predominantly expressed in the brain and are speculated to be involved in synaptic regulation and neuronal plasticity. α-Synuclein, identified as a component of Alzheimer’s disease amyloid plaques, is localized to neuronal cell bodies and synapses. Coordinate expression of α-synuclein and β-synuclein may be important during hematopoetic cell differentiation. A mutant form of α-synuclein is found in patients with early onset Parkinson’s disease. γ-Synuclein is associated with axonal pathology in Parkinson’s disease. |
| Immunogen | recombinant protein |
| Isotype | IgG |
|---|---|
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Alpha synuclein antibody Beta synuclein antibody NACP antibody Non A beta component of AD amyloid antibody PARK1 antibody PARK4 antibody PD1 antibody SNCA antibody SNCB antibody Synuclein alpha antibody Synuclein beta antibody |
|---|---|
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane, Secreted, Cell junction. |
Application
Fig1: Western blot analysis of alpha+beta Synuclein on different lysates with Rabbit anti-alpha+beta Synuclein antibody at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Rat brain tissue lysate, Lysates/proteins at 40 µg/Lane. Predicted band size: 14 kDa Observed band size: 15 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining alpha+beta Synuclein in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining alpha+beta Synuclein in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-alpha+beta Synuclein antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha+beta Synuclein antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with alpha+beta Synuclein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | N2A, SH-SY-5Y, Hela, rat brain tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
