Application
Fig1: Western blot analysis of MSI2 on different lysates using anti-MSI2 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: PC-12Application
Fig2: ICC staining MSI2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MSI2 in Lovo cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MSI2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-MSI2 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MSI2 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with MSI2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | MSI2 [ST59-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ36569 antibody MGC3245 antibody Msi2 antibody MSI2/HOXA9 fusion gene, included antibody MSI2H antibody MSI2H_HUMAN antibody Musashi 2 antibody Musashi homolog 2 antibody Musashi RNA binding protein 2 antibody Musashi, Drosophila, homolog of, 2 antibody Musashi-2 antibody RNA binding protein Musashi homolog 2 antibody RNA-binding protein Musashi homolog 2 antibody WD 40 repeat protein MSI2 antibody |
| Product description | Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to post-translational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ36569 antibody
MGC3245 antibody
Msi2 antibody
MSI2/HOXA9 fusion gene included antibody MSI2H antibody MSI2H_HUMAN antibody Musashi 2 antibody Musashi homolog 2 antibody Musashi RNA binding protein 2 antibody Musashi Drosophila homolog of 2 antibody Musashi-2 antibody RNA binding protein Musashi homolog 2 antibody RNA-binding protein Musashi homolog 2 antibody WD 40 repeat protein MSI2 antibody |
|---|---|
| Molecular Weight(MW) | 35/37 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of MSI2 on different lysates using anti-MSI2 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: PC-12
Application
Fig2: ICC staining MSI2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MSI2 in Lovo cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with MSI2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Lovo, PC-12, Hela, mouse prostate tissue, human liver tissue, mouse liver tissue, mouse heart tissue, human pancreas tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
