ICC/IF
ICC staining Histone H3(mono methyl R2) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Histone H3(mono methyl R2) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Histone H3(mono methyl R2) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H3 (mono methyl R2) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Product Name | Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Immunogen | Synthetic peptide within human Histone H3 aa 1-49/136. |
| Modification | mono methyl R2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ChIPICC/IFIHC |
| IHC:1:200 ICC/IF:1:50-1:200 ChIP:Use 0.5~2 μg for 25 μg of chromatin. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | HIST1 cluster H3J antibody Histone gene cluster 1 H3 histone family member E antibody Histone gene cluster 1 H3G antibody H3 histone family member J antibody HIST1 cluster H3E antibody HIST1 cluster H3I antibody Histone gene cluster 1 H3C antibody FLJ92264 antibody H3 histone family member A antibody H3 histone family member B antibody H3 histone family member C antibody H3 histone family member D antibody H3 histone family member F antibody H3 histone family member H antibody H3 histone family member I antibody H3 histone family member K antibody H3 histone family member L antibody H3 histone family member T antibody H3 histone family 3A antibody H3.1 antibody H3.3A antibody H3/a antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/l antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3 antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FF antibody H3FH antibody H3FI antibody H3FJ antibody H3FK antibody H3FL antibody HIST1 cluster H3A antibody HIST1 cluster H3B antibody HIST1 cluster H3C antibody HIST1 cluster H3D antibody HIST1 cluster H3F antibody HIST1 cluster H3G antibody HIST1 cluster H3H antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1 H3a antibody Histone 1 H3b antibody Histone 1 H3c antibody Histone 1 H3d antibody Histone 1 H3e antibody Histone 1 H3f antibody Histone 1 H3g antibody Histone 1 H3h antibody Histone 1 H3i antibody Histone 1 H3j antibody histone 3 H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1 H3a antibody Histone cluster 1 H3b antibody Histone cluster 1 H3c antibody Histone cluster 1 H3d antibody Histone cluster 1 H3e antibody Histone cluster 1 H3f antibody Histone cluster 1 H3g antibody Histone cluster 1 H3i antibody Histone cluster 1 H3j antibody Histone gene cluster 1 H3 histone family member A antibody Histone gene cluster 1 H3 histone family member B antibody Histone gene cluster 1 H3 histone family member C antibody Histone gene cluster 1 H3 histone family member D antibody Histone gene cluster 1 H3 histone family member F antibody Histone gene cluster 1 H3 histone family member G antibody Histone gene cluster 1 H3 histone family member H antibody Histone gene cluster 1 H3 histone family member I antibody Histone gene cluster 1 H3 histone family member J antibody Histone gene cluster 1 H3A antibody Histone gene cluster 1 H3B antibody Histone gene cluster 1 H3D antibody Histone gene cluster 1 H3E antibody Histone gene cluster 1 H3F antibody Histone gene cluster 1 H3H antibody Histone gene cluster 1 H3I antibody Histone gene cluster 1 H3J antibody Histone H 3 antibody Histone H3.1 antibody histone H3.1t antibody Histone H3.2 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody Histone H3/o antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Nucleus, Chromosome. |
ICC/IF
ICC staining Histone H3(mono methyl R2) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Histone H3(mono methyl R2) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Histone H3(mono methyl R2) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H3 (mono methyl R2) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Application Notes | IHC:1:200 ICC/IF:1:50-1:200 ChIP:Use 0.5~2 μg for 25 μg of chromatin. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
