Application
Fig1: Western blot analysis of JNK2 on SHG-44 cell lysates using anti-JNK2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining JNK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining JNK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining JNK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-JNK2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with JNK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | JNK2 [SC05-85] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | c Jun kinase 2 antibody C Jun N terminal kinase 2 antibody c-Jun N-terminal kinase 2 antibody JNK 55 antibody JNK-55 antibody JNK2 alpha antibody JNK2 antibody JNK2 beta antibody JNK2A antibody JNK2alpha antibody JNK2B antibody JNK2BETA antibody Jun kinase antibody MAP kinase 9 antibody MAPK 9 antibody Mapk9 antibody Mitogen activated protein kinase 9 antibody Mitogen-activated protein kinase 9 antibody MK09_HUMAN antibody P54a antibody p54aSAPK antibody PRKM9 antibody Protein kinase, mitogen-activated, 9 antibody SAPK alpha antibody SAPK antibody SAPK1a antibody Stress activated protein kinase 1a antibody Stress-activated protein kinase JNK2 antibody |
| Product description | c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1, and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2, and JNK3β2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | c Jun kinase 2 antibody
C Jun N terminal kinase 2 antibody
c-Jun N-terminal kinase 2 antibody
JNK 55 antibody
JNK-55 antibody
JNK2 alpha antibody
JNK2 antibody
JNK2 beta antibody
JNK2A antibody
JNK2alpha antibody
JNK2B antibody
JNK2BETA antibody
Jun kinase antibody
MAP kinase 9 antibody
MAPK 9 antibody
Mapk9 antibody
Mitogen activated protein kinase 9 antibody
Mitogen-activated protein kinase 9 antibody
MK09_HUMAN antibody
P54a antibody
p54aSAPK antibody
PRKM9 antibody
Protein kinase mitogen-activated 9 antibody SAPK alpha antibody SAPK antibody SAPK1a antibody Stress activated protein kinase 1a antibody Stress-activated protein kinase JNK2 antibody |
|---|---|
| Molecular Weight(MW) | 46/54 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of JNK2 on SHG-44 cell lysates using anti-JNK2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining JNK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining JNK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining JNK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-JNK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with JNK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SHG-44, MCF-7, Hela, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
