WB
Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: THP-1 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ATG7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin.IHC
\Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin.FC
Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ATG7 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy. |
| Immunogen | Synthetic peptide within Human ATG7 aa 654-703 / 703. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:1000 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 1810013K23Rik antibody
Apg 7 antibody
APG7 autophagy 7 like antibody
APG7 autophagy 7-like (S. cerevisiae) antibody
APG7 like antibody
APG7 S. cerevisiae homolog of antibody APG7-like antibody APG7L antibody ATG 7 antibody ATG12-activating enzyme E1 ATG7 antibody ATG7 antibody ATG7 autophagy related 7 homolog (S. cerevisiae) antibody ATG7 autophagy related 7 homolog antibody ATG7_HUMAN antibody Atg7l antibody Autophagy 7 S. cerevisiae homolog of antibody Autophagy related protein 7 antibody Autophagy-related 7 (yeast) antibody Autophagy-related protein 7 antibody DKFZp434N0735 antibody GSA 7 antibody GSA7 antibody hAGP7 antibody Ubiquitin activating enzyme E1 like protein antibody Ubiquitin-activating enzyme E1-like protein antibody Ubiquitin-like modifier-activating enzyme ATG7 antibody |
|---|---|
| Molecular Weight(MW) | 78 kDa(Observed band size: 70 kDa) |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O95352 |
|---|
WB
Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: THP-1 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ATG7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
IHC
\Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
FC
Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:1000 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
