Application
Fig1: Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: SW480Application
Fig2: ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | Daxx [SC54-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BING 2 antibody BING2 antibody CENP-C binding protein antibody DAP 6 antibody DAP6 antibody DAXX antibody DAXX_HUMAN antibody Death associated protein 6 antibody Death domain associated protein 6 antibody Death domain associated protein antibody Death domain-associated protein 6 antibody EAP 1 antibody EAP1 antibody ETS1 associated protein 1 antibody ETS1-associated protein 1 antibody Fas binding protein antibody Fas death domain associated protein antibody Fas death domain-associated protein antibody hDaxx antibody MGC126245 antibody MGC126246 antibody |
| Product description | Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BING 2 antibody BING2 antibody CENP-C binding protein antibody DAP 6 antibody DAP6 antibody DAXX antibody DAXX_HUMAN antibody Death associated protein 6 antibody Death domain associated protein 6 antibody Death domain associated protein antibody Death domain-associated protein 6 antibody EAP 1 antibody EAP1 antibody ETS1 associated protein 1 antibody ETS1-associated protein 1 antibody Fas binding protein antibody Fas death domain associated protein antibody Fas death domain-associated protein antibody hDaxx antibody MGC126245 antibody MGC126246 antibody |
|---|---|
| Molecular Weight(MW) | 81 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Chromosome. |
| SwissProt ID | Q9UER7 |
|---|
Application
Fig1: Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: SW480
Application
Fig2: ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | PANC-1, A549, Hela, SW480. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
