Application
Fig1: Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: THP-1 Lane 3: HelaApplication
Fig2: ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | MTCO2 [SC06-88] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COII antibody COX2 antibody COX2_HUMAN antibody COXII antibody Cytochrome c oxidase II antibody Cytochrome c oxidase polypeptide II antibody Cytochrome c oxidase subunit 2 antibody MT CO2 antibody MT-CO2 antibody MTCO2 antibody |
| Product description | Cytochrome c oxidase subunit II (COX2), also designated COII, MTCO2 or oxidative phosphorylation (OxPhos) complex IV, subunit II, is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electrochemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. Defects in COX2 are associated with tumor formation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | COII antibody COX2 antibody COX2_HUMAN antibody COXII antibody Cytochrome c oxidase II antibody Cytochrome c oxidase polypeptide II antibody Cytochrome c oxidase subunit 2 antibody MT CO2 antibody MT-CO2 antibody MTCO2 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Mitochondrion inner membrane. |
| SwissProt ID | P00403 |
|---|
Application
Fig1: Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: THP-1 Lane 3: Hela
Application
Fig2: ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | THP-1, MCF-7, Hela, HepG2, A549, human liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
