WB
Western blot analysis of C/EBP Beta on different lysates with Rabbit anti-C/EBP Beta antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: U-87 MG cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.FC
Flow cytometric analysis of C/EBP Beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | C/EBP Beta Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The C/EBP β form requires phosphorylation for its DNA binding ability, and increase binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification. |
| Immunogen | Synthetic peptide within Rat C/EBP Beta aa 248-297 / 297. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | AGP/EBP antibody C EBP beta antibody C/EBP beta antibody C/EBP related protein 2 antibody CCAAT Enhancer Binding Protein beta antibody CCAAT/enhancer-binding protein beta antibody CEBPB antibody CEBPB_HUMAN antibody CRP2 antibody IL 6DBP antibody IL6DBP antibody Interleukin 6 dependent binding protein antibody LAP antibody Liver activator protein antibody Liver enriched transcriptional activator antibody NF IL6 antibody NFIL6 antibody Nuclear factor NF IL6 antibody Nuclear factor NF-IL6 antibody SF B antibody SFB antibody Silencer factor B antibody TCF-5 antibody TCF5 antibody Transcription factor 5 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of C/EBP Beta on different lysates with Rabbit anti-C/EBP Beta antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: U-87 MG cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
FC
Flow cytometric analysis of C/EBP Beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:2000 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
