Application
Fig1: Western blot analysis of NQO2 on Hela cells lysates using anti-NQO2 antibody at 1/1,000 dilution.| Product Name | NQO2 [SC06-68] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DHQV antibody DIA6 antibody EC 1.10.99.2 antibody MGC94180 antibody NAD(P)H dehydrogenase quinone 2 antibody NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody NMOR2 antibody NQO 2 antibody NQO2 antibody NQO2_HUMAN antibody NRH dehydrogenase [quinone] 2 antibody NRH dehydrogenase antibody NRH:quinone oxidoreductase 2 antibody OTTHUMP00000015948 antibody OTTHUMP00000015949 antibody OTTHUMP00000015953 antibody Ox 2 antibody Ox2 antibody QR2 antibody Quinone antibody Quinone reductase 2 antibody Ribosyldihydronicotinamide dehydrogenase [quinone] antibody Ribosyldihydronicotinamide dehydrogenase antibody |
| Product description | NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity. NQO2 is a 231 amino acid protein and is 43 amino acids shorter than NQO1 at its C-terminus. NQO2 is an isozyme of NQO1 and transfers two electrons to a quinone, resulting in the formation of a hydroquinone product . The NQO2 gene is ubiquitously expressed and induced in response to TCDD. NQO2 has a higher level of expression in mouse liver and testis than NQO1, which is highly expressed in the heart. NQO2 has a different cofactor requirement than NQO1 and uses dihydronicotinamide riboside (NRH) rather than NAD(P)H as an electron donor. Unlike NQO1, NQO2 is resistant to typical inhibitors of NQO1 such as dicoumarol, Cibacron blue and phenindonee, but is inhibited by quercetin and benzo(a)pyrene. NQO2 contains a specific metal binding site, which is absent in NQO1 and several cis-elements including SP1 binding sites, CCAAT box, XRE and ARE, which are located at the NQO2 gene promoter. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:1,000-1:2,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DHQV antibody DIA6 antibody EC 1.10.99.2 antibody MGC94180 antibody NAD(P)H dehydrogenase quinone 2 antibody NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody NMOR2 antibody NQO 2 antibody NQO2 antibody NQO2_HUMAN antibody NRH dehydrogenase [quinone] 2 antibody NRH dehydrogenase antibody NRH:quinone oxidoreductase 2 antibody OTTHUMP00000015948 antibody OTTHUMP00000015949 antibody OTTHUMP00000015953 antibody Ox 2 antibody Ox2 antibody QR2 antibody Quinone antibody Quinone reductase 2 antibody Ribosyldihydronicotinamide dehydrogenase [quinone] antibody Ribosyldihydronicotinamide dehydrogenase antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of NQO2 on Hela cells lysates using anti-NQO2 antibody at 1/1,000 dilution.| Positive Control | Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
