WB
Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Rat heart tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Myosin Light Chain 2 antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Myosin Light Chain 2 antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
Myosin Light Chain 2 was immunoprecipitated in 0.2mg mouse heart lysate with Rabbit anti-Myosin Light Chain 2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Myosin Light Chain 2 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse heart lysate (input), Lane 2: Rabbit anti-Myosin Light Chain 2 antibody IP in mouse heart lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Myosin Light Chain 2 antibody in mouse heart lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 15 seconds.| Product Name | Myosin Light Chain 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Encoded by the MYL2 gene, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, also designated MLC-2 or MLC2v, is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult cardiac ventricle muscle. Myosin regulatory light chain 2 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 (MYL7) and myosin regulatory light chain 2 in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myocardium, permits the delineation of their boundaries. At the amino acid level there is 96% homology between the human and mouse myosin regulatory light chain sequences. Mutations in MYL2 are correlated with mid-left ventricular chamber type hypertrophic cardiomyopathy (MVC2) as well as familial hypertrophic cardiomyopathy type 10 (CMH10). |
| Immunogen | Synthetic peptide within Human Myosin Light Chain 2 aa 117-166 / 166. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:5000 IHC:1:800-4000 IP:Use at an assay dependent concentration. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Cardiac myosin light chain-2 antibody Cardiac ventricular myosin light chain 2 antibody CMH10 antibody MLC 2v antibody MLC-2 antibody MLC-2v antibody MLC2 antibody MLRV_HUMAN antibody MYL 2 antibody MYL2 antibody Myosin light chain 2 regulatory cardiac slow antibody Myosin light polypeptide 2 regulatory cardiac slow antibody Myosin regulatory light chain 2 antibody Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody Regulatory light chain of myosin antibody RLC of myosin antibody Slow cardiac myosin regulatory light chain 2 antibody ventricular/cardiac muscle isoform antibody |
|---|---|
| Molecular Weight(MW) | 19 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Rat heart tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Myosin Light Chain 2 antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Myosin Light Chain 2 antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
Myosin Light Chain 2 was immunoprecipitated in 0.2mg mouse heart lysate with Rabbit anti-Myosin Light Chain 2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Myosin Light Chain 2 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse heart lysate (input), Lane 2: Rabbit anti-Myosin Light Chain 2 antibody IP in mouse heart lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Myosin Light Chain 2 antibody in mouse heart lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 15 seconds.| Application Notes | WB:1:500-1:5000 IHC:1:800-4000 IP:Use at an assay dependent concentration. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
