WB
Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody at 1/1,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: HeLa cell lysate, Lane 3: K-562 cell lysate, Lane 4: C6 cell lysate, Lane 5: RAW264.7 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Tie-1 and Tie-2 (also designated Tek) are novel cell surface receptor tyrosine kinases. The extracellular domain of Tie-1 has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three fibronectin type III repeats next to the transmembrane region. Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2. Preliminary biochemical analyses of Ang-1 reveal a potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is an angiogenic factor that is thought to be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression seems to be restricted to sites of vascular remodeling. |
| Immunogen | Synthetic peptide within Human Angiopoietin 1 aa 249-300 / 498. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | AGP 1 antibody AGP1 antibody AGPT antibody ANG 1 antibody ANG antibody ANG-1 antibody ANG1 antibody Angiopoietin-1 antibody Angiopoietin1 antibody ANGP1_HUMAN antibody ANGPT 1 antibody Angpt1 antibody KIAA0003 antibody OTTHUMP00000227592 antibody OTTHUMP00000227599 antibody OTTHUMP00000227600 antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa(Observed band size: 65 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody at 1/1,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: HeLa cell lysate, Lane 3: K-562 cell lysate, Lane 4: C6 cell lysate, Lane 5: RAW264.7 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
