Application
Fig1: Western blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining AGR2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining AGR2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AGR2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with AGR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | AGR2 [SN74-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AG 2 antibody AG 2 protein antibody AG-2 antibody AG2 antibody AG2 protein antibody AGR 2 antibody AGR2 antibody AGR2_HUMAN antibody Anterior gradient 2 homolog (Xenopus laevis) antibody anterior gradient 2 homolog antibody Anterior gradient 2, Xenopus, homolog of antibody Anterior gradient homolog 2 antibody Anterior gradient protein 2 homolog antibody GOB 4 antibody GOB4 antibody HAG 2 antibody hAG-2 antibody hAG2 antibody HAG2/R antibody HPC 8 antibody hPC8 antibody PDIA17 antibody Protein disulfide isomerase family A member 17 antibody secreted cement gland homolog antibody Secreted cement gland protein XAG 2 homolog antibody Secreted cement gland protein XAG-2 homolog antibody Secreted cement gland protein XAG2 homolog antibody XAG 2 antibody XAG2 antibody |
| Product description | it is involved in cement gland differentiation and neural marker gene expression. AGR2 is a secretory protein encoded by two different AGR2 transcripts. It interacts with LYPD3 and α-dystroglycan (DAG-1). AGR2 is ubiquitously expressed with up-regulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR). AGR2 may serve as a potential therapeutic marker for various cancers. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AG 2 antibody
AG 2 protein antibody
AG-2 antibody
AG2 antibody
AG2 protein antibody
AGR 2 antibody
AGR2 antibody
AGR2_HUMAN antibody
Anterior gradient 2 homolog (Xenopus laevis) antibody
anterior gradient 2 homolog antibody
Anterior gradient 2 Xenopus homolog of antibody Anterior gradient homolog 2 antibody Anterior gradient protein 2 homolog antibody GOB 4 antibody GOB4 antibody HAG 2 antibody hAG-2 antibody hAG2 antibody HAG2/R antibody HPC 8 antibody hPC8 antibody PDIA17 antibody Protein disulfide isomerase family A member 17 antibody secreted cement gland homolog antibody Secreted cement gland protein XAG 2 homolog antibody Secreted cement gland protein XAG-2 homolog antibody Secreted cement gland protein XAG2 homolog antibody XAG 2 antibody XAG2 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Secreted, Endoplasmic reticulum. |
| SwissProt ID | O95994 |
|---|
Application
Fig1: Western blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining AGR2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining AGR2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AGR2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with AGR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NIH/3T3, MCF-7, Hela, human colon tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
