WB
Western blot analysis of FANCD2 on different lysates with Rabbit anti-FANCD2 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: K-562 cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: C6 cell lysate, Lane 5: Mouse testis tissue lysate, Lane 6: Rat testis tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1: 8 seconds; Lane 2-3: 30 seconds; Lane 4-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling FANCD2 with Rabbit anti-FANCD2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FANCD2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.| Product Name | FANCD2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Fanconi anemia (FA) is an autosomal recessive disorder character-ized by bone marrow failure, birth defects and chromosomal instability. At the cellular level, FA is characterized by spontaneous chromosomal breakage and a unique hypersensitivity to DNA cross-linking agents. At least 8 complementation groups (A-G) have been identified and 6 FA genes (for subtypes A, C, D2, E, F and G) have been cloned. The FA proteins lack sequence homologies or motifs that could point to a molecular function. Phosphorylation of FANC (Fanconi anemia complementation group) proteins are thought to be important for the function of the FA pathway. Several FA proteins, including FANCA, FANCC, FANCF, and FANCG, interact in a nuclear complex, and this complex is required for the activation (monoubiquitination) of the downstream FANCD2 protein. When monoubiquitinated, the FANCD2 protein co-localizes with the breast cancer susceptibility protein BRCA1 in DNA damage induced foci. In male meiosis, FANCD2 also co-localizes with BRCA1 at synaptonemal complexes. The human FANCD2 gene maps to chromosome 3p25.3, contains 44 exons and encodes a 1,451-amino acid nuclear protein that exists as 2 protein isoforms. |
| Immunogen | Synthetic peptide within Human FANCD2 aa 191-240 / 1,451. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | DKFZp762A223 antibody FA 4 antibody FA D2 antibody FA4 antibody FAC D2 antibody FACD 2 antibody FACD antibody FACD2 antibody FACD2_HUMAN antibody FAD antibody FAD2 antibody FANC D2 antibody FANCD 2 antibody FANCD antibody FANCD2 antibody FANCONI ANEMIA COMPLEMENTATION GROUP D antibody Fanconi anemia complementation group D2 antibody Fanconi anemia group D2 protein antibody FANCONI PANCYTOPENIA TYPE 4 antibody FLJ23826 antibody OTTHUMP00000158853 antibody OTTHUMP00000207925 antibody Protein FACD2 antibody Type 4 Fanconi pancytopenia antibody |
|---|---|
| Molecular Weight(MW) | 164 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
WB
Western blot analysis of FANCD2 on different lysates with Rabbit anti-FANCD2 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: K-562 cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: C6 cell lysate, Lane 5: Mouse testis tissue lysate, Lane 6: Rat testis tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1: 8 seconds; Lane 2-3: 30 seconds; Lane 4-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling FANCD2 with Rabbit anti-FANCD2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FANCD2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FANCD2 antibody. Counter stained with hematoxylin.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
