Application
Fig1: Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IRF5 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IRF5 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of K562 cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | IRF5 [SN201-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interferon regulatory factor 5 antibody Interferon regulatory factor 5 bone marrow variant antibody IRF 5 antibody IRF-5 antibody Irf5 antibody IRF5_HUMAN antibody SLEB10 antibody |
| Product description | Interferon regulatory factor 5 (IRF-5), belongs to the IRF family of DNA-binding factors, which includes IRF-1, IRF-2, IRF-3, IRF-4, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP). The IRF family regulate both interferon and interferon-inducible genes. IRF-5, like IRF-3 and IRF-7, is a direct transducer of virus-mediated signaling and plays a role in the expression of multiple cytokines/chemokines. Although IRF-5 is a direct target of p53, its cell cycle regulatory and proapoptotic effects are p53 independent. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Interferon regulatory factor 5 antibody Interferon regulatory factor 5 bone marrow variant antibody IRF 5 antibody IRF-5 antibody Irf5 antibody IRF5_HUMAN antibody SLEB10 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q13568 |
|---|
Application
Fig1: Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IRF5 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IRF5 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of K562 cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | THP-1, human lung cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
