Application
Fig1: Western blot analysis of Histone H1.0 on different lysates with Rabbit anti-Histone H1.0 antibody at 1/500 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: PC-3 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining Histone H1.0 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.| Product Name | Histone H1.0 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Immunogen | Synthetic peptide within Human Histone H10 aa 1-50 / 194. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | H1 histone family member 0 antibody H1(0) antibody H10 antibody H10_HUMAN antibody h1f0 antibody H1FV antibody Histone H1'' antibody Histone H1(0) antibody Histone H1.0 antibody Histone H10 antibody Histone H5 antibody MGC5241 antibody N-terminally processed antibody |
|---|---|
| Molecular Weight(MW) | 30kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: Western blot analysis of Histone H1.0 on different lysates with Rabbit anti-Histone H1.0 antibody at 1/500 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: PC-3 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining Histone H1.0 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, human tonsil tissue, human breast carcinoma tissue, human kidney tissue, mouse liver tissue, human pancreas tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:800 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
