Application
Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.| Product Name | 14-3-3 sigma [SD2070] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 14 3 3 protein sigma antibody 14-3-3 protein sigma antibody 1433S_HUMAN antibody Epithelial cell marker protein 1 antibody Er antibody HME 1 antibody HME1 antibody MGC143283 antibody Mkrn3 antibody Mme1 antibody OTTHUMP00000004242 antibody RP23 137L22.11 antibody SFN antibody SFN protein antibody Stratifin antibody YWHAS antibody |
| Product description | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | 14 3 3 protein sigma antibody 14-3-3 protein sigma antibody 1433S_HUMAN antibody Epithelial cell marker protein 1 antibody Er antibody HME 1 antibody HME1 antibody MGC143283 antibody Mkrn3 antibody Mme1 antibody OTTHUMP00000004242 antibody RP23 137L22.11 antibody SFN antibody SFN protein antibody Stratifin antibody YWHAS antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Secreted. |
Application
Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.| Positive Control | Human tonsil tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
