Application
Fig1: Western blot analysis of p53 (acetyl K382) on different lysates with Rabbit anti-p53 (acetyl K382) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 treated with 400nM TSA and 0.5μM doxorubicin for 24 hours whole cell lysate, Lane 2: NIH/3T3 whole cell lysate, Lane 3: HeLa treated with 400nM TSA and 0.5μM doxorubicin for 24 hours whole cell lysate, Lane 4: HeLa whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunocytochemistry analysis of HeLa cells untreated / treated with 400nM TSA and 0.5µM doxorubicin for 24 hours labeling p53 (acetyl K382) with Rabbit anti-p53 (acetyl K382) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K382) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.Application
Fig3: ICC staining p53 (acetyl K382) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p53 (acetyl K382) antibody. Counter stained with hematoxylin.| Product Name | p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody |
| Immunogen | Synthetic peptide within Human p53 aa 350 to the C-terminus (acetyl K382). |
| Modification | acetyl K382 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICCIHCWB |
| WB:1:1000-1:2000 ICC:1:50-1:100 IHC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ |
| Purification | Protein A |
| Alternative Names | Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix. |
| SwissProt ID | P04637 |
|---|
Application
Fig1: Western blot analysis of p53 (acetyl K382) on different lysates with Rabbit anti-p53 (acetyl K382) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 treated with 400nM TSA and 0.5μM doxorubicin for 24 hours whole cell lysate, Lane 2: NIH/3T3 whole cell lysate, Lane 3: HeLa treated with 400nM TSA and 0.5μM doxorubicin for 24 hours whole cell lysate, Lane 4: HeLa whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunocytochemistry analysis of HeLa cells untreated / treated with 400nM TSA and 0.5µM doxorubicin for 24 hours labeling p53 (acetyl K382) with Rabbit anti-p53 (acetyl K382) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K382) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Application
Fig3: ICC staining p53 (acetyl K382) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p53 (acetyl K382) antibody. Counter stained with hematoxylin.| Positive Control | A549, HepG2, human skin tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1000-1:2000 ICC:1:50-1:100 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
