WB
Western blot analysis of Phospho-AMPK alpha 1 (S496) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody at 1/5,000 dilution. Lane 1: SiHa cell lysate, Lane 2: HUVEC cell lysate, Lane 3: K-562 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-AMPK alpha 1(S496) on HUVEC cell lysates. Lane 1: HUVEC cells, whole cell lysate, 10ug/lane, Lane 2/3: HUVEC cells treated with UV(2 hours recovery), whole cell lysates, 10ug/lane, Lane 4: HUVEC cells treated with UV(2 hours recovery), then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-AMPK alpha 1(S496) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 14 seconds.IF-F
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody. The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).IF-F
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody. The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Phospho-AMPK alpha 1 (S496) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | AMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKα1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser496 of Human AMPK alpha 1 aa 471-520 / 559. |
| Modification | p-S496 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IF-FWB |
| WB:1:500-1:1000 IF-F:1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 5 AMP activated protein kinase alpha 1catalytic subunit antibody
5 AMP activated protein kinase catalytic alpha 1 chain antibody
5' AMP activated protein kinase catalytic subunit alpha 1 antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
AAPK1 antibody
AAPK1_HUMAN antibody
ACACA kinase antibody
acetyl CoA carboxylase kinase antibody
AI194361 antibody
AI450832 antibody
AL024255 antibody
AMP -activate kinase alpha 1 subunit antibody
AMP-activated protein kinase catalytic alpha -1 antibody AMPK 1 antibody AMPK alpha 1 antibody AMPK alpha 1 chain antibody AMPK antibody AMPK subunit alpha-1 antibody AMPK1 antibody AMPKa1 antibody AMPKalpha1 antibody C130083N04Rik antibody cb116 antibody EC 2.7.11.1 antibody HMG CoA reductase kinase antibody HMGCR kinase antibody hormone sensitive lipase kinase antibody Hydroxymethylglutaryl CoA reductase kinase antibody im:7154392 antibody kinase AMPK alpha1 antibody MGC33776 antibody MGC57364 antibody OTTHUMP00000161795 antibody OTTHUMP00000161796 antibody PRKAA 1 antibody PRKAA1 antibody Protein kinase AMP activated alpha 1 catalytic subunit antibody SNF1-like protein AMPK antibody SNF1A antibody Tau protein kinase PRKAA1 antibody wu:fa94c10 antibody |
|---|---|
| Molecular Weight(MW) | 64 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q13131 |
|---|
WB
Western blot analysis of Phospho-AMPK alpha 1 (S496) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody at 1/5,000 dilution. Lane 1: SiHa cell lysate, Lane 2: HUVEC cell lysate, Lane 3: K-562 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-AMPK alpha 1(S496) on HUVEC cell lysates. Lane 1: HUVEC cells, whole cell lysate, 10ug/lane, Lane 2/3: HUVEC cells treated with UV(2 hours recovery), whole cell lysates, 10ug/lane, Lane 4: HUVEC cells treated with UV(2 hours recovery), then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-AMPK alpha 1(S496) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 14 seconds.
IF-F
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody. The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
IF-F
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody. The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:500-1:1000 IF-F:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
