WB
Western blot analysis of Phospho-Raf1 (S43) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate, Lane 2: 293T cell lysate.| Product Name | Phospho-Raf1 (S43) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser43 of Human Raf1 aa 20-69 / 648. |
| Modification | p-S43 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | c Raf antibody
C-raf antibody
C-Raf proto-oncogene serine/threonine kinase antibody CMD1NN antibody Craf 1 transforming gene antibody cRaf antibody Craf1 transforming gene antibody EC 2.7.11.1 antibody kinase Raf1 antibody Murine sarcoma 3611 oncogene 1 antibody NS5 antibody Oncogene MIL antibody Oncogene RAF1 antibody OTTHUMP00000160218 antibody OTTHUMP00000207813 antibody OTTHUMP00000209389 antibody Protein kinase raf 1 antibody Proto-oncogene c-RAF antibody Raf 1 antibody Raf 1 proto oncogene serine/threonine kinase antibody RAF antibody Raf proto oncogene serine/threonine protein kinase antibody RAF proto-oncogene serine/threonine-protein kinase antibody RAF-1 antibody RAF1 antibody RAF1_HUMAN antibody Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody v raf 1 murine leukemia viral oncogene homolog 1 antibody v-raf murine sarcoma viral oncogene homolog 1 antibody v-raf-1 murine leukemia viral oncogene-like protein 1 antibody vraf1 murine leukemia viral oncogene homolog 1 antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cytoplasm, Cell membrane, Mitochondrion, Nucleus. |
WB
Western blot analysis of Phospho-Raf1 (S43) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate, Lane 2: 293T cell lysate.| Positive Control | NIH/3T3, 293T. |
|---|---|
| Application Notes | WB:1:500-1:2000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
