Application
Fig1: Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5,000 dilution.Application
Fig2: ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining UBA1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining UBA1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of K562 cells with UBA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Ubiquitin-like modifier-activating enzyme 1 [SD08-62] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A1S9 antibody A1S9 protein antibody A1S9T and BN75 temperature sensitivity complementing antibody A1S9T antibody A1ST antibody AMCX1 antibody CFAP124 antibody CTD-2522E6.1 antibody GXP 1 antibody GXP1 antibody MGC4781 antibody POC20 antibody POC20 centriolar protein homolog antibody Protein A1S9 antibody SMAX2 antibody Uba1 antibody UBA1, ubiquitin-activating enzyme E1 homolog A antibody UBA1_HUMAN antibody UBA1A antibody UBE 1 antibody UBE 1X antibody UBE1 antibody UBE1X antibody Ubiquitin activating enzyme E1 antibody Ubiquitin-activating enzyme E1 antibody Ubiquitin-like modifier-activating enzyme 1 antibody |
| Product description | The ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. Specifically, UBE1 functions to adenylate the C-terminal glycine residue of ubiquitin, a reaction that is ATP-dependent and is proceeded by the formation of a thiolester bond with a cysteine residue of UBE1. The UBE1-activated ubiquitin is then transferred to a ubiquitin conjugated enzyme, which donates the ubiquitin residue to target substrates. The UBE1 gene is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. However, no UBE1 homolog is detectable on the human Y chromosome. UBE1 is thought to escape X inactivation in humans. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | A1S9 antibody
A1S9 protein antibody
A1S9T and BN75 temperature sensitivity complementing antibody
A1S9T antibody
A1ST antibody
AMCX1 antibody
CFAP124 antibody
CTD-2522E6.1 antibody
GXP 1 antibody
GXP1 antibody
MGC4781 antibody
POC20 antibody
POC20 centriolar protein homolog antibody
Protein A1S9 antibody
SMAX2 antibody
Uba1 antibody
UBA1 ubiquitin-activating enzyme E1 homolog A antibody UBA1_HUMAN antibody UBA1A antibody UBE 1 antibody UBE 1X antibody UBE1 antibody UBE1X antibody Ubiquitin activating enzyme E1 antibody Ubiquitin-activating enzyme E1 antibody Ubiquitin-like modifier-activating enzyme 1 antibody |
|---|---|
| Molecular Weight(MW) | 118 kDa |
| Cellular Localization | Cytoplasm, Mitochondrion, Nucleus. |
Application
Fig1: Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5,000 dilution.
Application
Fig2: ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining UBA1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining UBA1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of K562 cells with UBA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SKOV-3, A549, Hela, human kidney tissue, mouse liver tissue, mouse ovary tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
