Application
Fig1: Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Cdc23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cdc23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | CDC23 [SD08-60] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANAPC8 antibody Anaphase promoting complex subunit 8 antibody Anaphase-promoting complex subunit 8 antibody Apc 8 antibody APC8 antibody Cdc 23 antibody CDC23 antibody CDC23_HUMAN antibody cell division cycle 23 antibody Cell division cycle 23 homolog antibody Cell division cycle protein 23 antibody Cell division cycle protein 23 homolog antibody Cut23 antibody Cyclosome subunit 8 antibody |
| Product description | Cell cycle progression is controlled at a point late in G1 designated Start. Passage through Start requires the activity of the cyclin-dependent protein kinase Cdc28. Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family. Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC). This complex consists of two proteins, Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle. Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids. The APC is thought to be stabilized by Cdc26 (also known as Scd26). In addition to these APC proteins, Cdc5 is also required for completion of mitosis. In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ANAPC8 antibody Anaphase promoting complex subunit 8 antibody Anaphase-promoting complex subunit 8 antibody Apc 8 antibody APC8 antibody Cdc 23 antibody CDC23 antibody CDC23_HUMAN antibody cell division cycle 23 antibody Cell division cycle 23 homolog antibody Cell division cycle protein 23 antibody Cell division cycle protein 23 homolog antibody Cut23 antibody Cyclosome subunit 8 antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa |
| Cellular Localization | Cytosol. |
Application
Fig1: Western blot analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Cdc23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cdc23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
