Application
Fig1: Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7Application
Fig2: ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining 14-3-3 Theta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining 14-3-3 Theta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 Theta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | 14-3-3 Theta [SD084-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 14-3-3 antibody 14-3-3 protein T-cell antibody 14-3-3 protein tau antibody 14-3-3 protein theta antibody 1C5 antibody HS1 antibody theta polypeptide antibody tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein antibody tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta isoform antibody tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody |
| Product description | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 14-3-3 antibody
14-3-3 protein T-cell antibody
14-3-3 protein tau antibody
14-3-3 protein theta antibody
1C5 antibody
HS1 antibody
theta polypeptide antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta isoform antibody tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta polypeptide antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7
Application
Fig2: ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining 14-3-3 Theta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining 14-3-3 Theta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 Theta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, MCF-7, Hela, A549. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
