Application
Fig1: Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: JurkatApplication
Fig2: ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cdc34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cdc34 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Cdc34 [JJ086-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdc 34 antibody Cdc34 antibody Cell division cycle 34 antibody Cell division cycle 34 homolog (S. cerevisiae) antibody Cell division cycle 34 homolog antibody E2 CDC34 antibody UB2R1_HUMAN antibody UBC 3 antibody UBC3 antibody UBCH3 antibody UBE2 R1 antibody UBE2R1 antibody Ubiquitin carrier protein antibody Ubiquitin conjugating enzyme Cdc34 antibody Ubiquitin conjugating enzyme E2 32 kDa complementing antibody Ubiquitin protein ligase antibody Ubiquitin protein ligase R1 antibody Ubiquitin-conjugating enzyme E2 R1 antibody Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody Ubiquitin-conjugating enzyme E2-CDC34 antibody Ubiquitin-protein ligase R1 antibody |
| Product description | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIPFC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cdc 34 antibody Cdc34 antibody Cell division cycle 34 antibody Cell division cycle 34 homolog (S. cerevisiae) antibody Cell division cycle 34 homolog antibody E2 CDC34 antibody UB2R1_HUMAN antibody UBC 3 antibody UBC3 antibody UBCH3 antibody UBE2 R1 antibody UBE2R1 antibody Ubiquitin carrier protein antibody Ubiquitin conjugating enzyme Cdc34 antibody Ubiquitin conjugating enzyme E2 32 kDa complementing antibody Ubiquitin protein ligase antibody Ubiquitin protein ligase R1 antibody Ubiquitin-conjugating enzyme E2 R1 antibody Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody Ubiquitin-conjugating enzyme E2-CDC34 antibody Ubiquitin-protein ligase R1 antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Jurkat
Application
Fig2: ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cdc34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cdc34 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, N2A, Hela, K562, Jurkat. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
