WB
Western blot analysis of Phospho-Raf1(S621) on human skeletal muscle lysates using anti-Phospho-Raf1(S621) antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded rat trachea tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Phospho-Raf1 (S621) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser621 of Human Raf1 aa 591-640 / 648. |
| Modification | p-S621 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:2000 IHC:1:100-1:500 |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | c Raf antibody
C-raf antibody
C-Raf proto-oncogene serine/threonine kinase antibody CMD1NN antibody Craf 1 transforming gene antibody cRaf antibody Craf1 transforming gene antibody EC 2.7.11.1 antibody kinase Raf1 antibody Murine sarcoma 3611 oncogene 1 antibody NS5 antibody Oncogene MIL antibody Oncogene RAF1 antibody OTTHUMP00000160218 antibody OTTHUMP00000207813 antibody OTTHUMP00000209389 antibody Protein kinase raf 1 antibody Proto-oncogene c-RAF antibody Raf 1 antibody Raf 1 proto oncogene serine/threonine kinase antibody RAF antibody Raf proto oncogene serine/threonine protein kinase antibody RAF proto-oncogene serine/threonine-protein kinase antibody RAF-1 antibody RAF1 antibody RAF1_HUMAN antibody Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody v raf 1 murine leukemia viral oncogene homolog 1 antibody v-raf murine sarcoma viral oncogene homolog 1 antibody v-raf-1 murine leukemia viral oncogene-like protein 1 antibody vraf1 murine leukemia viral oncogene homolog 1 antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cytoplasm, Cell membrane, Mitochondrion, Nucleus. |
WB
Western blot analysis of Phospho-Raf1(S621) on human skeletal muscle lysates using anti-Phospho-Raf1(S621) antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded rat trachea tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:2000 IHC:1:100-1:500 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
