Application
Fig1: Western blot analysis of HDAC6 on Jurkat cells lysates using anti-HDAC6 antibody at 1/1,000 dilution.Application
Fig2: ICC staining HDAC6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HDAC6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining HDAC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC6 antibody. Counter stained with hematoxylin.| Product Name | HDAC6 [JJ09-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CPBHM antibody FLJ16239 antibody HD 6 antibody HD6 antibody HDAC 6 antibody HDAC6 antibody HDAC6_HUMAN antibody Histone deacetylase 6 (HD6) antibody Histone deacetylase 6 antibody JM 21 antibody JM21 antibody KIAA0901 antibody OTTHUMP00000032398 antibody OTTHUMP00000197663 antibody PPP1R90 antibody Protein phosphatase 1 regulatory subunit 90 antibody |
| Product description | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CPBHM antibody FLJ16239 antibody HD 6 antibody HD6 antibody HDAC 6 antibody HDAC6 antibody HDAC6_HUMAN antibody Histone deacetylase 6 (HD6) antibody Histone deacetylase 6 antibody JM 21 antibody JM21 antibody KIAA0901 antibody OTTHUMP00000032398 antibody OTTHUMP00000197663 antibody PPP1R90 antibody Protein phosphatase 1 regulatory subunit 90 antibody |
|---|---|
| Molecular Weight(MW) | 131 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Perikaryon, Cell projection. |
Application
Fig1: Western blot analysis of HDAC6 on Jurkat cells lysates using anti-HDAC6 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining HDAC6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HDAC6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining HDAC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC6 antibody. Counter stained with hematoxylin.| Positive Control | 293, Jurkat, Hela, HepG2, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
