WB
Western blot analysis of Estrogen Receptor beta on different lysates with Rabbit anti-Estrogen Receptor beta antibody at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate (low expression), Lane 2: Daudi cell lysate, Lane 3: MDA-MB-468 cell lysate (low expression), Lane 4: SK-Br-3 cell lysate (low expression), Lane 5: MCF7 cell lysate (low expression), Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Estrogen Receptor beta antibody at 1/150 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/150 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Estrogen Receptor beta Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. |
| Immunogen | Recombinant protein within Human Estrogen Receptor beta aa 440-530 / 530. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:2000 IHC:1:150 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | ER BETA antibody ER-beta antibody Erb antibody ESR B antibody ESR BETA antibody ESR2 antibody ESR2_HUMAN antibody ESRB antibody ESTRB antibody estrogen nuclear receptor beta variant a antibody estrogen nuclear receptor beta variant b antibody estrogen receptor 2 (ER beta) antibody Estrogen receptor 2 antibody estrogen receptor beta 4 antibody Estrogen receptor beta antibody NR3A2 antibody Nuclear receptor subfamily 3 group A member 2 antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa(Observed band size: 55 kDa) |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q92731 |
|---|
WB
Western blot analysis of Estrogen Receptor beta on different lysates with Rabbit anti-Estrogen Receptor beta antibody at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate (low expression), Lane 2: Daudi cell lysate, Lane 3: MDA-MB-468 cell lysate (low expression), Lane 4: SK-Br-3 cell lysate (low expression), Lane 5: MCF7 cell lysate (low expression), Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Estrogen Receptor beta antibody at 1/150 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/150 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:2000 IHC:1:150 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
