Application
Fig1: Western blot analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: JurkatApplication
Fig2: ICC staining CDC5L in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CDC5L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CDC5L in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining CDC5L in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: ICC staining CDC5L in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig7: ICC staining CDC5L in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CDC5L antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CDC5L antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CDC5L antibody. Counter stained with hematoxylin.Application
Fig11: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CDC5L antibody. Counter stained with hematoxylin.| Product Name | CDC5L [JJ091-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC5 antibody CDC5 cell division cycle 5-like (S. pombe) antibody Cdc5 related protein antibody CDC5-LIKE antibody Cdc5-like protein antibody Cdc5l antibody CDC5L_HUMAN antibody CEF1 antibody Cell division cycle 5 S. pombe homolog of antibody Cell division cycle 5-like protein antibody dJ319D22.1 antibody KIAA0432 antibody PCDC5RP antibody Pombe Cdc5 related protein antibody Pombe Cdc5-related protein antibody |
| Product description | Cdc5L (Cell division cycle 5-like protein, Pombe Cdc5-related protein) is a DNA-binding protein encoded by the human gene CDC5L. Cdc5L contains 2 HTH myb-type DNA-binding domains and may shuttle between cytoplasm and nucleus. It is involved in cell cycle control and may act as a transcription activator. CDC5L is a spliceosomal protein that is highly conserved across species. It is a member of a protein group that comprise the core of spliceosomal complexes and are essential for pre-mRNA splicing. Cdc5L is involved in the second catalytic step of pre-mRNA splicing, which involves cleavage at the 3' splice site and the ligation of the exons. This process releases the intact intron lariat. A chromosomal aberration involving Cdc5L is found in multicystic renal dysplasia. This aberration is caused by a translocation with USF-2. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CDC5 antibody CDC5 cell division cycle 5-like (S. pombe) antibody Cdc5 related protein antibody CDC5-LIKE antibody Cdc5-like protein antibody Cdc5l antibody CDC5L_HUMAN antibody CEF1 antibody Cell division cycle 5 S. pombe homolog of antibody Cell division cycle 5-like protein antibody dJ319D22.1 antibody KIAA0432 antibody PCDC5RP antibody Pombe Cdc5 related protein antibody Pombe Cdc5-related protein antibody |
|---|---|
| Molecular Weight(MW) | 92/100 kDa |
| Cellular Localization | Nucleus, Nucleus speckle, Cytoplasm. |
Application
Fig1: Western blot analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat
Application
Fig2: ICC staining CDC5L in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CDC5L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CDC5L in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining CDC5L in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: ICC staining CDC5L in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig7: ICC staining CDC5L in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CDC5L antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CDC5L antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CDC5L antibody. Counter stained with hematoxylin.
Application
Fig11: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CDC5L antibody. Counter stained with hematoxylin.| Positive Control | Jurkat, MCF-7, Hela, HepG2, NIH/3T3, 293T, SH-SY-5Y, human kidney tissue, mouse lung tissue, mouse kidney tissue, mouse stomach tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
