Application
Fig1: ICC staining SDF1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining SDF1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining SDF1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | SDF1 [JJ09-20] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody AI174028 antibody C-X-C motif chemokine 12 antibody Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody Chemokine (C-X-C motif) ligand 12 antibody Chemokine CXC motif ligand 12 antibody cxcl12 antibody hIRH antibody hSDF-1 antibody Intercrine reduced in hepatomas antibody IRH antibody OTTHUMP00000019491 antibody PBSF antibody Pre-B cell growth-stimulating factor antibody SCYB12 antibody SDF 1 antibody SDF-1 antibody SDF-1-alpha(3-67) antibody SDF-1a antibody SDF-1b antibody SDF1_HUMAN antibody SDF1A antibody SDF1B antibody Stromal cell-derived factor 1 antibody Stromal cell-derived factor 1 delta antibody Stromal cell-derived factor 1 gamma antibody Stromal cell-derived factor 1a antibody Stromal cell-derived factor-1 alpha antibody Thymic lymphoma cell-stimulating factor antibody Tlsf antibody TLSF-a antibody TLSF-b antibody Tlsfa antibody Tlsfb antibody TPAR1 antibody |
| Product description | The stromal cell derived factor 1 (SDF1, CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors. The SDF1 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells. SDF1 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells. Many cancer cells express CXCR4, suggesting that SDF1 plays a role in cancer metastasis. Alternative splicing and differential processing during maturation produce a pair of SDF1 isoforms (SDF1α and SDF1β) that have different properties and biological activities. Additional isoforms of SDF1 have been reported. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody AI174028 antibody C-X-C motif chemokine 12 antibody Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody Chemokine (C-X-C motif) ligand 12 antibody Chemokine CXC motif ligand 12 antibody cxcl12 antibody hIRH antibody hSDF-1 antibody Intercrine reduced in hepatomas antibody IRH antibody OTTHUMP00000019491 antibody PBSF antibody Pre-B cell growth-stimulating factor antibody SCYB12 antibody SDF 1 antibody SDF-1 antibody SDF-1-alpha(3-67) antibody SDF-1a antibody SDF-1b antibody SDF1_HUMAN antibody SDF1A antibody SDF1B antibody Stromal cell-derived factor 1 antibody Stromal cell-derived factor 1 delta antibody Stromal cell-derived factor 1 gamma antibody Stromal cell-derived factor 1a antibody Stromal cell-derived factor-1 alpha antibody Thymic lymphoma cell-stimulating factor antibody Tlsf antibody TLSF-a antibody TLSF-b antibody Tlsfa antibody Tlsfb antibody TPAR1 antibody |
|---|---|
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P48061 |
|---|
Application
Fig1: ICC staining SDF1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining SDF1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining SDF1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | SH-SY-5Y, SW480, 293. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
