WB
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: A431 cell lysate (15 µg/Lane), Lane 3: NIH/3T3 cell lysate (15 µg/Lane), Lane 4: PC-12 cell lysate (15 µg/Lane), Lane 5: Mouse brain tissue lysate (20 µg/Lane), Lane 6: Mouse colon tissue lysate (20 µg/Lane), Lane 7: Rat brain tissue lysate (20 µg/Lane), Lane 8: Rat colon tissue lysate (20 µg/Lane), Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.IF-P
Species: Human, Site: Colon carcinoma, Sample: Paraffin-embedded section, Antibody concentration: 1/100.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Actin with Rabbit anti-Actin antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Actin antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | Actin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | le structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| Immunogen | Synthetic peptide within Human Actin aa 45-80 / 377. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IF-PWB |
| WB:1:10000-1:50000 IF-P:1:100-1:500 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRatZebrafish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | a actin antibody
ACTA antibody
ACTA1 antibody
Actin alpha skeletal muscle antibody
Actin antibody
actin alpha 1 skeletal muscle 1 antibody actin alpha 1 skeletal muscle antibody Actin alpha skeletal muscle antibody actina antibody actine antibody ACTS_HUMAN antibody aktin antibody Alpha Actin 1 antibody Alpha skeletal muscle Actin antibody alpha skeletal muscle antibody alpha-actin antibody Alpha-actin-1 antibody ASMA antibody CFTD antibody CFTD1 antibody CFTDM antibody MPFD antibody NEM1 antibody NEM2 antibody NEM3 antibody nemaline myopathy type 3 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: A431 cell lysate (15 µg/Lane), Lane 3: NIH/3T3 cell lysate (15 µg/Lane), Lane 4: PC-12 cell lysate (15 µg/Lane), Lane 5: Mouse brain tissue lysate (20 µg/Lane), Lane 6: Mouse colon tissue lysate (20 µg/Lane), Lane 7: Rat brain tissue lysate (20 µg/Lane), Lane 8: Rat colon tissue lysate (20 µg/Lane), Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
IF-P
Species: Human, Site: Colon carcinoma, Sample: Paraffin-embedded section, Antibody concentration: 1/100.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Actin with Rabbit anti-Actin antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Actin antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:10000-1:50000 IF-P:1:100-1:500 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
