Application
Fig1: Western blot analysis of Glutathione Peroxidase 1 on different lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: THP-1| Product Name | Glutathione Peroxidase 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IPWB |
| WB:1:1,000-1:2,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | AL033363 antibody Cellular glutathione peroxidase antibody Glutathione peroxidase 1 antibody Glutathione peroxidase antibody GPx 1 antibody GPx-1 antibody GPX1 antibody GPX1_HUMAN antibody GPXD antibody GSHPx-1 antibody GSHPX1 antibody MGC14399 antibody MGC88245 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Glutathione Peroxidase 1 on different lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: THP-1| Positive Control | HUVEC, MCF-7, Hela, HepG2, human liver tissue, mouse liver tissue, mouse brain tissue, human lung tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
