WB
Western blot analysis of PKC delta on different lysates with Rabbit anti-PKC delta antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: Jurkat cell lysate (15 µg/Lane), Lane 3: Mouse brain tissue lysate (20 µg/Lane), Lane 4: Rat brain tissue lysate (20 µg/Lane), Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of RH-35 cells labeling PKC delta with Rabbit anti-PKC delta antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PKC delta antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.| Product Name | PKC delta Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca;2+;. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Immunogen | Recombinant protein within mouse PKC delta aa 525-671 / 676. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CVID9 antibody D14Ertd420e antibody Kinase PKC delta antibody KPCD antibody KPCD_HUMAN antibody MAY 1 antibody MAY1 antibody MGC49908 antibody nPKC delta antibody nPKC-delta antibody PCKd antibody PKC d antibody PKC delta antibody PKCD antibody PKCdelta antibody PRKC D antibody PRKC delta antibody Prkcd antibody Protein Kinase C delta antibody Protein kinase C delta type antibody Protein kinase C delta VIII antibody Protein Kinase Cdelta antibody Tyrosine protein kinase PRKCD antibody |
|---|---|
| Molecular Weight(MW) | 78 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane. |
WB
Western blot analysis of PKC delta on different lysates with Rabbit anti-PKC delta antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: Jurkat cell lysate (15 µg/Lane), Lane 3: Mouse brain tissue lysate (20 µg/Lane), Lane 4: Rat brain tissue lysate (20 µg/Lane), Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of RH-35 cells labeling PKC delta with Rabbit anti-PKC delta antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PKC delta antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.| Application Notes | WB:1:2000-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
