Application
Fig1: Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.| Product Name | EBP50 [JJ0946] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EBP 50 antibody EBP50 antibody Ezrin radixin moesin binding phosphoprotein 50 antibody Ezrin-radixin-moesin-binding phosphoprotein 50 antibody Na(+)/H(+) exchange regulatory cofactor NHE RF antibody Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody Na+/H+ exchange regulatory co factor antibody NHERF 1 antibody NHERF antibody NHERF-1 antibody NHERF1 antibody NHRF1_HUMAN antibody NPHLOP2 antibody Regulatory cofactor of Na(+)/H(+) exchanger antibody Slc9a3r1 antibody Sodium hydrogen exchanger regulatory factor 1 antibody Sodium-hydrogen exchanger regulatory factor 1 antibody Sodium/hydrogen exchanger regulatory factor 1 antibody Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody Solute carrier family 9 isoform 3 regulatory factor 1 antibody Solute carrier family 9 isoform A3 regulatory factor 1 antibody Solute carrier family 9 member 3 regulator 1 antibody |
| Product description | The Na+/H+ exchange protein (NHE3) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE3 regulatory factor 1 (NHERF-1) interacts with NHE3 through two PDZ (for PSD-95, Discs-large and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific carboxy-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE3 to decrease Na+ adsorption. NHERF-1 functions as a scaffold for an essential multiprotein complex of Ezrin and NHE3 for cAMP-mediated phosphorylation and consequent inhibition of NHE3. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction. NHERF-2, also known as E3KARP, is a ubiquitously expressed protein which also functions in NHE2 regulation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | EBP 50 antibody
EBP50 antibody
Ezrin radixin moesin binding phosphoprotein 50 antibody
Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
Na+/H+ exchange regulatory co factor antibody
NHERF 1 antibody
NHERF antibody
NHERF-1 antibody
NHERF1 antibody
NHRF1_HUMAN antibody
NPHLOP2 antibody
Regulatory cofactor of Na(+)/H(+) exchanger antibody
Slc9a3r1 antibody
Sodium hydrogen exchanger regulatory factor 1 antibody
Sodium-hydrogen exchanger regulatory factor 1 antibody
Sodium/hydrogen exchanger regulatory factor 1 antibody
Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulatory factor 1 antibody Solute carrier family 9 isoform 3 regulatory factor 1 antibody Solute carrier family 9 isoform A3 regulatory factor 1 antibody Solute carrier family 9 member 3 regulator 1 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm, Apical cell membrane, Endomembrane system, Cell projection. |
Application
Fig1: Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, rat kidney tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
