Application
Fig1: Western blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: SKOV-3Application
Fig2: ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HE4 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of SW480 cells with HE4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | HE4 [JF62-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | dJ461P17.6 antibody EDDM4 antibody epididymal protein 4 antibody Epididymal secretory protein E4 antibody Epididymis specific whey acidic protein type four disulfide core antibody HE 4 antibody Major epididymis specific protein E4 antibody Major epididymis-specific protein E4 antibody MGC57529 antibody Putative protease inhibitor WAP5 antibody WAP 5 antibody WAP domain containing protein HE4 antibody WAP domain containing protein HE4 V4 antibody WAP four disulfide core domain 2 antibody WAP four disulfide core domain protein 2 antibody WAP four-disulfide core domain protein 2 antibody WAP5 antibody WFDC 2 antibody WFDC2 antibody WFDC2_HUMAN antibody |
| Product description | HE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | dJ461P17.6 antibody EDDM4 antibody epididymal protein 4 antibody Epididymal secretory protein E4 antibody Epididymis specific whey acidic protein type four disulfide core antibody HE 4 antibody Major epididymis specific protein E4 antibody Major epididymis-specific protein E4 antibody MGC57529 antibody Putative protease inhibitor WAP5 antibody WAP 5 antibody WAP domain containing protein HE4 antibody WAP domain containing protein HE4 V4 antibody WAP four disulfide core domain 2 antibody WAP four disulfide core domain protein 2 antibody WAP four-disulfide core domain protein 2 antibody WAP5 antibody WFDC 2 antibody WFDC2 antibody WFDC2_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: SKOV-3
Application
Fig2: ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HE4 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of SW480 cells with HE4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SKOV-3, SW480, human lung tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
