WB
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody at 1/50,000 dilution. Lane 1: HeLa cell lysate, Lane 2: L-929 cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: C6 cell lysate, Lane 5: COS-1 cell lysate, Lane 6: Rat heart tissue lysate, Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight.| Product Name | HRP Conjugated GAPDH Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the β-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins. |
| Immunogen | Recombinant full length protein of Human GAPDH aa 1-335 / 335. |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:50000 |
|
| Species Reactivity | ChickenFishHumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 38 kDa BFA-dependent ADP-ribosylation substrate antibody
aging associated gene 9 protein antibody
Aging-associated gene 9 protein antibody
BARS-38 antibody
cb609 antibody
EC 1.2.1.12 antibody
Epididymis secretory sperm binding protein Li 162eP antibody
G3P_HUMAN antibody
G3PD antibody
G3PDH antibody
GAPD antibody
GAPDH antibody
Glyceraldehyde 3 phosphate dehydrogenase antibody
Glyceraldehyde-3-phosphate dehydrogenase antibody
HEL-S-162eP antibody
KNC-NDS6 antibody
MGC102544 antibody
MGC102546 antibody
MGC103190 antibody
MGC103191 antibody
MGC105239 antibody
MGC127711 antibody
MGC88685 antibody
OCAS p38 component antibody OCT1 coactivator in S phase 38-KD component antibody peptidyl cysteine S nitrosylase GAPDH antibody Peptidyl-cysteine S-nitrosylase GAPDH antibody wu:fb33a10 antibody |
|---|---|
| Molecular Weight(MW) | 36kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane. |
WB
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody at 1/50,000 dilution. Lane 1: HeLa cell lysate, Lane 2: L-929 cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: C6 cell lysate, Lane 5: COS-1 cell lysate, Lane 6: Rat heart tissue lysate, Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight.| Application Notes | WB:1:50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
